Differentiation of Microorganisms Lab Report Example | Topics and Well Written Essays - 1000 words

Differentiation of Microorganisms - Lab Report Example The researcher tells that in culture media experiment the test included a synthetic medium, tryptic soy broth, SF broth, Macconkey Agar, and nutrient agar. The liquid media like the nutrient broth was used in the reproduction and isolation of organisms. McConkey Agar was a solid medium that examined colony morphology and the isolation of the organisms. Most of the culture media were complex; their chemical composition was unknown. Additionally, the culture media were selective. They selected certain microbe types by supplying the growth conditions. An example of this media was SF medium; it was designed to select Enterococcus faecallis in warm-blooded animals. In Enzymes experiment, the tests included the Caseinase which hydrolyzed casein to peptides to amino acids, gelatinase hydrolyzed gelatin, and   Catalase protected the cells from the hydrogen peroxide. In addition, Oxidase indicated the presence of cytochrome in the microorganisms, during oxidation the compounds turned red or blue. In fermentation experiment, the fermentable compounds were sugar (glucose, sucrose, and lactose). Consequently, an indicator dye was used to change the color from red to a yellow color when the PH is below 7. In motility, experiment flagellum was used a bacterial motility. The experiment used several ways to determine motility i.e. hanging drop and motility agar. In the course of the experiment, if the culture tested is strict aerobe, then growth will occur on the tube surface. The hanging drop slide was used in motility viewing when the oxygen is depleted. In the experiment that dealt with differentiation of enteric bacteria, most bacteria used were facultative anaerobes and anaerobes. The enteric bacteria used here encompassed facultative anaerobic rods, gram negative, and non-spore forming bacteria. The Coliforms were characterized by their fermenting ability. Other tests that were used to identify the enteric bacteria included H2S, KCN, MB agar, TSI agar, SS agar, and MacConkey agar. Discussion The culture media experiment used the following microorganism: Escherichis Coli, Enterococcus faecalis, and Pseudomonas aeruginosa. The two culture media in the experiment were synthetic medium and tryptic Soy Broth. The

A Casualty Clearing Station Essay Example | Topics and Well Written Essays - 1000 words

A Casualty Clearing Station - Essay Example Dr. Hayward started by explaining how the conditions at war were not as one expected them to be but they were rather tougher and difficult to cope up with. To support this stand, he began his narrative by stating that he had worked for twenty years as a surgeon in the general hospitals in an area and he also had an exposure to war like situations as he had served as a surgeon at British Red Cross Hospital, Netley during the initial period of the War. But the author explains in his narrative that this familiarity with war like situations was in no way close to the experiences that he gained at the Casualty Clearing Station (C.C.S) at Crouay where he got posted upon his request in July 1918. This was because the C.C.S received patients directly from the war field and this was different from his working at the British Red Cross Hospital. Thus, Dr. Hayward provided an inside picture of the C.C.S by beginning his compilation from his normal days of work and ending it after his work at the C.C.S. The firsthand account of Dr. John A. Hayward is a text that provides historians with not only geographical evidence but also with historical and cultural evidence. From the geographical perspective, his writing narrates the war experiences of the different regions in France beginning with the Base Hospital at Trouville where he only dealt with minor injuries. This was followed by his work at the C.C.S at Crouay where he treated patients who were pouring in from the battlefield.